Figure 4.

Human suppressor genes rescue FUS toxicity upon overexpression. W303 MATα containing the empty vector, pRS416Gal1ccdB, or each of the identified human gene that suppress FUS toxicity were crossed with the FUS model generated in the isogenic strain with the opposite mating type, W303 MATa. Diploid yeast strains were selected, serially diluted and spotted onto agar plates containing glucose (genes-off condition) and galactose (genes-on condition), respectively. The picture, representing three independent experiments, was taken after growth at 30° for three days. *indicating different ORF clones of the same gene.