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The effect of ligand affinity, target expression, and target/protein type on yield and enrichment. Mixtures of binding and non-binding yeast were sorted in parallel by cell suspension and adherent panning and binding yeast yield, non-displaying yeast yield, and enrichment ratio were quantified. (A-C) Ligand: E6.2.6’ Cell Line: MDA-MB-231 (D-F) Ligand: AASV Cell Line: A431 (G-I) Ligand: AC2 Cell Line MS1-CD276
