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Stitching tiles along sample depth (z-axis). (a) Placement and orientation of connected tiles along depth. The top view of the tile is highlighted by a rectangle. (b) Virtual xy slices along the depth of the stitched volume of a thick mouse brain section. Some neurons at >350 μm depth (see Visualization 4) are resolved, with neuronal processes imaged at more superficial depths. (Scale bar: 100 μm)
