Fig. 3.

Wheat PBF TILLING mutants. Two wheat PBF triple mutant (aabbdd) lines were created by combining single A, B and D-homoeolog TILLING mutations in a single plant. Line 1 = Cadenza1533 x Cadenza0903 x Cadenza0904 (A missense x B nonsense x D nonsense). Line 2 = Cadenza1807 x Cadenza0903 x Cadenza0904 (A missense x B nonsense x D nonsense). Line 2A and 2B were derived from different F₁ grains.

A. A diagram of the PBF A, B and D gene structures. The exons are shown as bars and intron as lines. The coding regions are black and the DNA-binding DOF domain is red. The positions of the mutations are indicated by arrows. The two missense mutations are separated by 5 bp. C = Cadenza, NS = nonsense, MS = missense. B. Representative wild type (AABBDD) and mutant (aabbdd) F₃ grains, and grains of the non-mutant, parental cultivar Cadenza are shown. All five grains of each type are from a single plant. All F₂ plants were grown as a single batch. C. Representative wild type (AABBDD) and mutant (aabbdd) embryos are shown. Embryos are from F₃ grains, as shown in Fig. 3B.