KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| goat anti-GFP | Rockland Immunochemicals | 600-101-215 |
| rabbit anti-βGal | Thermo | PI31887 |
| rabbit anti-DsRed | Rockland Immunochemicals | 600-401-379 |
| mouse P1 (P1a+P1b) | From I. Ando lab (Kurucz et al., 2007b) | N/A |
| rabbit anti-Crq | From C. Kocks (Franc et al., 1996) | N/A |
| rabbit anti-Srp | A. Giangrande | N/A |
| donkey anti-goat Alexa 488 | Molecular probes | A-11055 |
| donkey anti-rabbit Alexa 488 | Molecular probes | A-21206 |
| donkey anti-mouse Alexa 488 | Molecular probes | A-21202 |
| Bacterial and Virus Strains | ||
| Escherichia coli | From B. Lemaitre lab | N/A |
| Micrococcus luteus | From B. Lemaitre lab | N/A |
| Erwinia carotovora carotovora 15 | From B. Lemaitre lab, (Lemaitre et al., 1997) | N/A |
| Enterobacter cloacae | β12, Jean Lambert, from B. Lemaitre lab, (Lemaitre et al., 1997)) | N/A |
| Serratia marcescens | From D. Ferrandon, (Nehme et al., 2007) | N/A |
| Biological Samples | ||
| N/A | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| OCT | Tissue-Tek | 4583 |
| Paraformaldehyde | Electron Microscopy Sciences | 15710 |
| Triton-X100 | Sigma | 50-904-5759 |
| Schneider’s medium | Gibco, Millipore | 21720024 |
| Complete protease inhibitor tablets | Roche | 04719948001 |
| AEBSF | Sigma | SBR00015 |
| Fluorescently labeled phalloidin | Molecular Probes | A34055 |
| DAPI | Sigma | D9542 |
| DRAQ5 | ThermoFisher | 62252 |
| OilRedO | Sigma | O1391 |
| Triethylphosphate | Sigma | 414050 |
| LipidTOX dye | LifeTech | H34350 |
| EdU | Invitrogen | E10415 |
| FluoSpheres carboxylate modified 0.2mm | Life Technologies | F8781 |
| E. coli K-12 Strain Bioparticles TexasRed Conjugate | Invitrogen | E2863 |
| pHrodo E. coli Bioparticles | Invitrogen | P35361 |
| TRIzol | Invitrogen | 10296–010 |
| Critical Commercial Assays | ||
| Click-IT EdU Cell proliferation assay | Invitrogen | C10214 |
| iScript cDNA synthesis kit | BioRad | 1708891 |
| Direct-zol RNA MiniPrep kit | Zymo Research | R2072 |
| BioRad iTaq SYBR Green Supermix | BioRad | 1725120 |
| TaKaRa DNA Ligation Kit | Takara Bio Inc | 6024 |
| Deposited Data | ||
| N/A | ||
| Experimental Models: Cell Lines | ||
| N/A | ||
| Experimental Models: Organisms/Strains | ||
| See Table S1 | ||
| Oligonucleotides | ||
| See Table S2 | ||
| Recombinant DNA | ||
| pFucci-S/G2/M Green cloning vector | MBL laboratories | AM-V9014 |
| pFucci-G1 Orange cloning vector | MBL laboratories | AM-V9001 |
| p-Red H-Stinger-HmlΔ-DsRed | Clark et al 2011 | N/A |
| HmlΔFucci-G1-Orange | This paper | N/A |
| HmlΔFucciGreenS/G2/M-Green | This paper | N/A |
| Software and Algorithms | ||
| Fiji image processing software | (Schindelin et al., 2012) | https://imagej.net/Fiji |
| Imaris Microscopy image analysis software | Oxford Instruments | N/A |
| Leica Application Suite (LAS) Microscopy Software | Leica Microsystems | N/A |
| Excel | Microsoft Office | N/A |
| Prism 8 | Graphpad | N/A |
| Other | ||
| N/A | ||