Table 2.

Microfluidic RT-PCR and Western Blot Analyses in Soleus Muscle

	Microfluidic RT-PCR Analyses (Relative mRNA Levels: Fold Change/Baseline)		Statistics
	Control (n = 8)	Protein (n = 9)	BR Effect	Trt Effect	BR*Trt Effect
IRS1	0.68 ± 0.09	0.95 ± 0.17	0.06	0.19	0.15
PIK3R1	0.95 ± 0.11	1.12 ± 0.11	0.71	0.28	0.31
PIK3R2	1.04 ± 0.15	1.24 ± 0.07	0.11	0.26	0.26
PKB	0.96 ± 0.13	0.91 ± 0.06	0.31	0.79	0.70
GSK3B	0.96 ± 0.08	1.01 ± 0.07	0.74	0.58	0.61
PRKCA	1.08 ± 0.18	0.96 ± 0.09	0.84	0.74	0.53
GLUT4	0.87 ± 0.15	0.89 ± 0.09	0.15	0.94	0.90
MAP2K1	1.05 ± 0.15	0.96 ± 0.09	0.95	0.62	0.62
MAPK1	1.02 ± 0.14	0.98 ± 0.07	0.96	0.96	0.81

Data are means ± SEM.

Abbreviations: GLUT4, glucose transporter type 4; GSK3B, glycogen synthase kinase 3 beta; IRS1, insulin receptor 1; MAP2K1, mitogen-activated protein kinase kinase 1; MAPK1, mitogen-activated protein kinase 1; PIK3R1, phosphoinositide-3 kinase regulatory subunit 1; PIK3R2, rhosphoinositide-3 kinase regulatory subunit 2; PKB, protein kinase B; pPKB, phosphorylated protein kinase B; PRKCA, protein kinase C-alpha.