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. 2020 Jun 1;15(6):e0233695. doi: 10.1371/journal.pone.0233695

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© 2020 Hemati et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) Colony PCR for MAP mce-truncated gene, Lane M: 1kb DNA ladder (#SM0313, Fermentas), Lane 1: Positive control (MAP Indian Bison type DNA), Lane 2: Negative control (Nuclease Free Water), Lane 4: Negative in colony PCR for MAP Mce-truncated genes, Lanes 3 and 5–12 positive in colony PCR for MAP Mce-truncated genes; (B) Restriction digestion of confirmed colony PCR positive PTZ57R/T mce-truncated clones: Lane M: 1kb DNA ladder (#SM0313, Fermentas), Lane 2: Double digested pET28a plasmid, Lanes 4 and 6: Positive clones of PTZ57R/T mce-truncated plasmid; (C) Restriction digestion of confirmed colony PCR positive pET28a mce-truncated clones; Lane M: 1kb DNA ladder (RBC), lanes 1 and 2: Positive clones of PTZ57R/T mce-truncated plasmid.