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. 2020 Jun 1;15(6):e0233695. doi: 10.1371/journal.pone.0233695

Fig 3. Optimization condition for Mce-truncated protein expression.

Fig 3

(A) SDS-PAGE analysis of Mce-truncated protein expression level with different IPTG concentration at 200 C in E. coli strain BL21 (DE3) pLysS. (B) SDS-PAGE analysis of Mce-truncated protein production at different time intervals on the optimized concentration of IPTG (1.5mM IPTG). Lane M. Molecular weight marker (MBT092-100LN Hi-Media). Arrow indicates Mce-truncated protein.