Figure 8. Loss of Shm2p and Ade3p impinges on multiple cell cycle phases but in distinct ways.
(A) Double shm2Δ,ade3Δ deletion mutants are slow-growing. Two representative tetrad dissections from shm2Δ x ade3Δ crosses are shown. The yellow diamond indicates the shm2Δ,ade3Δ segregants. All the strains used in B-E were segregants from the same shm2Δ x ade3Δ crosses and, except as indicated, isogenic otherwise. (B) Violin plots of the mean and birth size of the indicated strains, calculated from ≥12 asynchronous cultures in each case. The plots were generated with the lattice R language package. Significant differences in pair-wise comparisons were indicated by the non-parametric Kruskal-Wallis test, while the p-values shown were for all significant differences based on the posthoc Nemenyi test (performed with the PMCMR R language package). (C) Representative DNA content histograms from the indicated strains, from at least 10,000 cells and ≥5 independent asynchronous cultures in each case. On the x-axis is fluorescence per cell, while the cell number is on the y-axis. The average and sd of the percentage of cells with unreplicated DNA (%G1) is shown. There were no statistically significant differences among the strains, based on the non-parametric Kruskal-Wallis test. (D) The rate of cell size increase (k, in h−1, shown on the y-axis) was calculated as described in Soma et al., 2014, for the strains shown on the x-axis. Each data point is the average of two technical replicates, from synchronous elutriated cultures. (E) The critical size (y-axis) for the strains shown on the x-axis was calculated as described in Soma et al., 2014, from the same cultures as in D.
Figure 8—figure supplement 1. The translational efficiency of SHM2 is cell cycle-regulated.
