Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (S. cerevisiae) | BY4743 | Giaever et al., 2002 | RRID:SCR_003093 | MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 |
| Strain, strain background (S. cerevisiae) | 32672 | Giaever et al., 2002 | RRID:SCR_003093 | rpl22aΔ::KanMX/rpl22aΔ::KanMX, BY4743 otherwise |
| Strain, strain background (S. cerevisiae) | 35844 | Giaever et al., 2002 | RRID:SCR_003093 | rpl22bΔ::KanMX/rpl22bΔ::KanMX, BY4743 otherwise |
| Strain, strain background (S. cerevisiae) | 30192 | Giaever et al., 2002 | RRID:SCR_003093 | rpl34aΔ::KanMX/rpl34aΔ::KanMX, BY4743 otherwise |
| Strain, strain background (S. cerevisiae) | 31445 | Giaever et al., 2002 | RRID:SCR_003093 | rpl34bΔ::KanMX/rpl34bΔ::KanMX, BY4743 otherwise |
| Strain, strain background (S. cerevisiae) | BY4742 | Steffen et al., 2012 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | |
| Strain, strain background (S. cerevisiae) | KS976 | Steffen et al., 2012 | rpl22aΔ::URA3, BY4742 otherwise | |
| Strain, strain background (S. cerevisiae) | KS979 | Steffen et al., 2012 | rpl22bΔ::URA3, BY4742 otherwise | |
| Strain, strain background (S. cerevisiae) | KS999 | Steffen et al., 2012 | rpl22a,bΔ::URA3, BY4742 otherwise | |
| Strain, strain background (S. cerevisiae) | BY4741 | Giaever et al., 2002 | RRID:SCR_003093 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
| Strain, strain background (S. cerevisiae) | MET3-TAP | Dharmacon | YSC1178-202231887 | MET3-TAP::HIS3M × 6, BY4741 otherwise |
| Strain, strain background (S. cerevisiae) | HB147 | This study | rpl22aΔ::URA3, MET3-TAP::HIS3M × 6, BY4741 otherwise | |
| Strain, strain background (S. cerevisiae) | HB171 | This study | rpl22bΔ::URA3, MET3-TAP::HIS3M × 6, BY4741 otherwise | |
| Strain, strain background (S. cerevisiae) | 13403 | Giaever et al., 2002 | RRID:SCR_003093 | shm1Δ::KanMX, BY4742 otherwise |
| Strain, strain background (S. cerevisiae) | 12669 | Giaever et al., 2002 | RRID:SCR_003093 | shm2Δ::KanMX, BY4742 otherwise |
| Strain, strain background (S. cerevisiae) | 16561 | Giaever et al., 2002 | RRID:SCR_003093 | ade17Δ::KanMX, BY4742 otherwise |
| Strain, strain background (S. cerevisiae) | 12384 | Giaever et al., 2002 | RRID:SCR_003093 | ade2Δ::KanMX, BY4742 otherwise |
| Strain, strain background (S. cerevisiae) | 6591 | Giaever et al., 2002 | RRID:SCR_003093 | ade3Δ::KanMX, BY4741 otherwise |
| Strain, strain background (S. cerevisiae) | NM64 | This study | MATα, shm2Δ::KanMX, ade3Δ::KanMX, met -, lys- | |
| Strain, strain background (S. cerevisiae) | NM65 | This study | MATa, shm2Δ::KanMX, ade3Δ::KanMX, met -, lys- | |
| Strain, strain background (S. cerevisiae) | NM66 | This study | MATα, ade17Δ::KanMX, rpl22aΔ::URA, his-, lys-, leu- | |
| Strain, strain background (S. cerevisiae) | NM67 | This study | MATα, shm2Δ::KanMX, rpl22aΔ::URA, his-, leu-, met- | |
| Strain, strain background (S. cerevisiae) | NM68 | This study | MATα, shm1Δ::KanMX, rpl22aΔ::URA, his-, leu-, met- | |
| Strain, strain background (S. cerevisiae) | BW885 | This study | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | |
| Other | Yeast extract | Sigma-Aldrich | Y1625 | |
| Other | Peptone | Sigma-Aldrich | P5905 | |
| Chemical compound, drug | Dextrose | Sigma-Aldrich | D9434 | |
| Chemical compound, drug | Cycloheximide | Calbiochem | 239763 M | |
| Chemical compound, drug | Sodium azide | Sigma-Aldrich | S2002 | |
| Chemical compound, drug | Tris(hydroxymethyl)aminomethane | Sigma-Aldrich | 252859 | |
| Chemical compound, drug | Tris base | Roche | TRIS-RO | |
| Chemical compound, drug | Sodium chloride | Sigma-Aldrich | S7653 | |
| Chemical compound, drug | Magnesium chloride hexahydrate | USP | 1374248 | |
| Chemical compound, drug | DTT | Sigma-Aldrich | D0632 | |
| Chemical compound, drug | Triton X-100 | Sigma-Aldrich | T8787 | |
| Peptide, recombinant protein | Turbo DNase I | ThermoFisher | AM2238 | |
| Other | Glass beads | Scientific Industries | SI-BG05 | |
| Other | 13 × 51 mm polycarbonate centrifuge tubes | Beckman Coulter | 349622 | |
| Chemical compound, drug | Sucrose | Sigma-Aldrich | S0389 | |
| Chemical compound, drug | Phosphate buffered saline (PBS) | Sigma-Aldrich | P4417 | |
| Commercial assay or kit | Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit | ThermoFisher | C10428 | |
| Chemical compound, drug | DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) | ThermoFisher | D1306 | |
| Commercial assay or kit | Ribo-Zero Magnetic Gold Kit (Yeast) | Epicentre | MRZY1324 | |
| Commercial assay or kit | SciptSeq v2 RNA-Seq Library Preparation Kit | Epicentre | SSV21124 | |
| Antibody | Peroxidase Anti-Peroxidase (PAP) Soluble Complex | Sigma-Aldrich | P1291 | (1:1000) |
| Antibody | Anti-Pgk1p antibody, rabbit polyclonal | abcam | ab38007 | (1:1000) |
| Other | NovexWedgeWell4–12% Tris-Glycine gels | ThermoFisher | XP04125 | |
| Software, algorithm | MetaboAnalyst | https://www.metaboanalyst.ca/ | RRID:SCR_015539 | Web server for statistical, functional and integrative analysis of metabolomics data |
| Software, algorithm | AccuComp Z2 | Beckman Coulter | 383550 | Software to monitor number and size of cells with Z2 cell counter |
| Software, algorithm | NIS-Elements | https://www.nikoninstruments.com/Products/Software | RRID:SCR_014329 | Microscope imaging software suite used with Nikon products |
| Software, algorithm | ImageJ | https://imagej.net/ | RRID:SCR_003070 | Image processing software |
| Software, algorithm | Adobe Photoshop | https://www.adobe.com/products/photoshop.html | RRID:SCR_014199 | Image processing software |
| Software, algorithm | RStudio | http://www.rstudio.com/ | RRID:SCR_000432 | Software for the R statistical computing environment |
| Software, algorithm | SGD | http://www.yeastgenome.org/ | RRID:SCR_004694 | Saccharomyces Genome Database |
| Software, algorithm | R | https://www.r-project.org | v3.5.2 RRID:SCR_001905 | Statistical Computing Environment |
| Software, algorithm | PANTHER | http://www.geneontology.org/ | RRID:SCR_002811 | Gene ontology enrichment analysis |