Figure 6.

Upregulation of FOXM1 eliminated the anti-tumor effect of miR-877-5p in HGC-27 cells. (A) Immunoblotting was performed to detect FOXM1 level in HGC-27 when treating with FOXM1 plasmid either with combination of miR-877-5p or vector counterparts. (B) Cell proliferation curve was drawn according to the results of CCK-8 assay in each time points among different groups. (C) The distribution of experimental groups in each cell cycle was measured using flow cytometry. (D) Apoptotic cell proportion when transfected miR-877-5p or vector with FOXM1 plasmid in HGC-27 cells, applying flow cytometry detection. Bar charts located on the bottom presented the details percentage of cells in each cell cycle phase (left) and apoptotic cells rate (right) when different combination of co-transfected items were conducted. *P < 0.05 and **P < 0.01.