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. 2020 May 26;12:3915–3925. doi: 10.2147/CMAR.S240719

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© 2020 Wang et al.

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CircMTO1 regulates cell proliferation and apoptosis in hepatoma cells. (A) Overexpression of circMTO1 in HepG2 and Hep3B cells transfected with pcDNA-circMTO1. (B) CCK-8 assay results showing proliferation rates of pcDNA-circMTO1 and NC cells. (C) Transwell assay showing the percentage of migrating cells in the pcDNA-circMTO1 and NC groups. (D) Flow cytometry plots showing percentage of AnnexinV+/PI+ apoptotic cells in both groups. **P<0.01, ***P<0.001 compared with NC group.

Abbreviations: NC, negative control; FITC, fluorescein isothiocyanate; PI, propidium iodide; FL1-H, fluorescent channel 1; FL2-H, fluorescent channel 2.