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. 2020 May 26;12:3915–3925. doi: 10.2147/CMAR.S240719

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© 2020 Wang et al.

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MiR-9-5p and circMTO1 mutually inhibit each other in hepatocarcinoma cells. (A) Putative binding sites between miR-9-5p and circMTO1. (B) Fluorescein activity in cells co-transfected with pmir-GLO-CircMTO1 3ʹUTR wt/mut and miR-9-5p mimic. (C, D) Results of qRT-PCR analysis showing up-regulation of miR-9-5p in (C) liver tumors and (D) HepG2 and Hep3B cells. (E) MiR-9-5p levels in hepatoma cells overexpressing circMTO1. (F) CircMTO1 levels in cells overexpressing miR-9-5p. **P<0.01 compared with NC group, ^##P<0.01 compared with the adjacent normal tissues, ^&&P<0.01 compared with L-02 cells.

Abbreviations: wt, wild type; mut, mutant type; NC, negative control.