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. 2020 May 26;12:3915–3925. doi: 10.2147/CMAR.S240719

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© 2020 Wang et al.

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MiR-9-5p targets NOX4 in hepatocarcinoma cells. (A) Binding sites between miR-9-5p and NOX4. (B) Luciferase activity in cells co-transfected with pmitr-GLO-NOX4 3ʹUTR wt/mut and miR-9-5p mimic. (C) NOX4 mRNA levels in the tumor and normal liver tissues. (D) In situ NOX4 expression in the tumor and normal liver tissues. (E) NOX4 mRNA levels in HepG2, Hep3B and L-02 cells. (F) NOX4 mRNA levels in cells transfected with miR-9-5p mimic or inhibitor. **P<0.01 compared with NC, ^##P<0.01 compared with adjacent normal tissues, ^&&P<0.01 compared with L-02.

Abbreviations: wt, wild type; mut, mutant type; NC, negative control.