Illustration of common structural changes associated with cryptic binding site formation. a. Side chain rearrangements as shown in IL2 (apo: 1M47_A, holo: 1PY2_D), b. loop movements in NPC2 (apo: 1NEP_A, holo: 2HKA_C), c. secondary structure changes like the partial helix unfolding in LfrR (apo: 2WGB_A, holo: 2V57_A), and d. interdomain motions in EF-TU (apo: 1EXM_A, holo: 1HA3_B). Apo structures are show in gray and holo structures in slate blue, while red arrows highlight the main structural changes.