Table 2. List of primers used in this study.

Primer names	Detection method	Sequence (5′-3′)	Location (nt)	Purpose	Size (bp)	References
CPV-F1	PCR	AGATAGTAATAATACTATGCCATTT	531-1250	Detection	719	[28]
CPV-R2		TTTTGAATCCAATCTCCTTCTGGAT
CaKV-F	RT-PCR	CCCTGGAACACCCAAGGCCGCT	-	Detection	504	[29]
CaKV-R		TCTGGTTGCCATAGATGTGGTG
CDV-F	RT-PCR	ACTTTCTGGCACTGTGATT	-	Detection	669	[29]
CDV-R		ACACCTTCAATNGCATGNAT
Herpesvirus type 1F	PCR	CTATGTTTCTTATGGATATGAGACTTTGTGAT	359-665	Detection	307	[30]
Herpesvirus type 1R		ATAGTTTTAACATTTCGACACCATTCATGTAG
CPV-1F	PCR	CAACGTAATGAGAACTAT	1-1253a	Genome sequencing	1,253	In this study
CPV-1R		CCCACAGCTTGTGCTATGGC
CPV-2F	PCR	GACAAGGTGGTAAAAGAAATACAG	1163-2445	Genome sequencing	1283	In this study
CPV-2R		ATGAGGTGGTGGTCTACTTC
CPV-3F	PCR	GAGCTAAAAAGGCAATTGCTCC	2345-3644	Genome sequencing	1,300	In this study
CPV-3R		CTTGGATCACCATCTGCTGC
CPV-4F	PCR	TGAGGCGTCTACACAAGGGC	3549-4269	Genome sequencing	721	In this study
CPV-4F		TTAGTATAATTTTCTAGGTGCTAGTT
FPV-1F	PCR	CTTTAGAACCAACTGACCAA	1-1419b	Genome sequencing	1,419	In this study
FPV-1R		CACATGCTATAGCGTGACAAAC
FPV-2F	PCR	GAATGCACGGATGGAATTGG	1372-2827	Genome sequencing	1456	In this study
FPV-2R		CTGACAGCAGGTTGACCACC
FPV-3F	PCR	AAAAGCCGGTGCAGGACAAG	2738-4240	Genome sequencing	1,503	In this study
FPV-3R		GGTGCATTTACATGAAGTCTTGG
FPV-4F	PCR	CCACCAGTTTATCCAAATGGTC	4161-4837	Genome sequencing	677	In this study
FPV-4R		CATATTCTAAGGGCAAACCAAC

CPV, canine parvovirus, CDV, canine distemper virus, CaKoV, canine kobuvirus; PCR, polymerase chain reaction; RT-PCR, reverse transcription polymerase chain reaction.

^aSite location, reference, GenBank accession number KR002799; ^bSite location, reference, GenBank accession number MH559110.