Fig. 5. The expression of identified critical proteins in exosomes was validated by qRT-PCR. The RNA expression of ITGα1 (A) and ARPC5 (C) was significantly decreased, while that of NCKAP1 (B) and TRIM62 (D) was significantly increased in exosomes from coinfected CEF cells compared with monoinfected cells. Exo-N represent exosomes from un-infected cells, Exo-R represent exosomes from REV infected cells; Exo-J represent exosomes from ALV-J infected cells; Exo-RJ represent exosomes from REV and ALV-J co-infected cells.

qRT-PCR, quantitative reverse transcription polymerase chain reaction; ITGα1, integrinα1; ARPC5, actin-related 2/3 complex subunit 5; NCKAP1, NCK-association proteins 1; TRIM62, tripartite motif containing 62; ALV-J, avian leukosis virus subgroup J; REV, reticuloendotheliosis virus.

^*p < 0.05; ^**p < 0.01; ^***p < 0.001.