Fig. 7. TRIM62 regulation of proteins involved in the actin cytoskeleton pathway. CEF cells were transfected with plasmid containing TRIM62 or empty vector for 12 h before single infection or coinfection with ALV-J and REV (A-C). CEF cells were transfected with shTRIM62 or empty vector for 24 h before infection (D-F). After 72 h of infection, the expression levels of ITGα1 (A, D), NCKAP1 (B, E), and ARPC5 (C, F) were measured. The black columns mean cells transfected with pTRIM62 (A-C) and with shTRIM62 (D-F); the grey columns mean cells transfected with empty vector (A-F).

TRIM62, tripartite motif containing 62; CEF, chicken embryo fibroblast; ALV-J, avian leukosis virus subgroup J; REV, reticuloendotheliosis virus; ITGα1, integrinα1; NCKAP1, NCK-association proteins 1.

^*p < 0.05; ^**p < 0.01; ^***p < 0.001.