. 2020 May 8;21(3):e49. doi: 10.4142/jvs.2020.21.e49

Table 1. Primers used to detect miRNA expression using qRT-PCR.

Gene target	Primer sequence	Fragment size (bp)
ALV-J (env)	Forward: TGCGTGCGTGGTTATTATTTC	144
ALV-J (env)	Reverse: AATGGTGAGGTCGCTGACTGT	144
REV (env)	Forward: TTGTTGAAGGCAAGCATCAG	330
REV (env)	Reverse: GAGGATAGCATCTGCCCTTT	330
TRIM62	Forward: TACTGGGAGGTGGTGGTGTC	246
TRIM62	Reverse: CGTCGGCGTTGTAGAAGATG	246
ITGα1	Forward: TAAGTTCATAGCGAGCGACC	125
ITGα1	Reverse: TCAGCACAG CCCCAT TCC	125
NCKAP1	Forward: TTGTCTTTTCGGTCGTTG	126
NCKAP1	Reverse: TGCCACCTTCATGTCAGT	126
ARPC5	Forward: TGGACGAGTACGACGAGA	254
ARPC5	Reverse: TGAGGACCTTCAGGA	254
GAPDH	Forward: GAACATCATCCCAGCGTCCA	132
GAPDH	Reverse: GAGGATAGCATCTGCCCTTT	132

qRT-PCR, quantitative reverse transcription polymerase chain reaction; ALV-J, avian leukosis virus subgroup J; REV, reticuloendotheliosis virus; TRIM62, tripartite motif containing 62; ITGα1, integrinα1; NCKAP1, NCK-association proteins 1; ARPC5, actin-related 2/3 complex subunit 5; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.