Fig. 6. Co-stimulation of PM2.5 and Pseudomonas aeruginosa activated signaling pathways. (A) Detection of NF-κB p65 and phospho-NF-κB p65 proteins expression levels in lung by western blot and the ratio of phospho-NF-κB p65 protein/NF-κB p65 (n = 3). Data are presented as the means ± SEM. (B) Detection of NF-κB p65 and phospho-NF-κB p65 protein expression levels in RAW264.7 cells by western blot and the ratio of phospho-NF-κB p65 protein/NF-κB p65 (n = 3). Data are presented as the means ± SEM.

PBS, phosphate-buffered saline; PM2.5, particulate matter less than 2.5 µm in diameter; NF, nuclear factor; SEM, standard error of the mean.

^*p < 0.05, ^**p < 0.01.