Fig. 2. Intracytoplasmic sperm injection. (A) MII-stage oocytes are held so that the first polar body is positioned at 12 o'clock and a single sperm (aspirated tail first) is pushed to the tip of the injection pipet (arrow). (B) The injection pipet is insert into oocyte cytoplasm through the zona pellucida at 3 o'clock.
MII-stage oocytes, mature oocytes at the metaphase II status.