Fig. 8. The highlights of superovulation and picking surrogate mother in female cynomolgus monkeys in this study. Firstly, sperms were collected from adult male cynomolgus monkey and oocytes were acquired by personalized superovulation protocol incorporated the menstrual cycle length of each cynomolgus monkey. After ICSI, the MII-stage oocytes could develop into embryos successfully. Then the 2–4-cell embryos were transplanted into the oviducts of the surrogate mothers selected according to the “menstrual cycles,” “sexual swelling,” “ovulation point,” and the pregnancy was successful. At last, the cynomolgus monkey fetus was acquired.

ICSI, intracytoplasmic sperm injection; MII-stage oocytes, mature oocytes at the metaphase II status.