Table 2. The 20 STRs locus and primers used in this study.
Locus | Rhesus chromosome location | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Fluorescent dye |
---|---|---|---|---|
1-D1S548* | 1 | GAACTCATTGGCAAAAGGAA | GCCTCTTTGTTGCAGTGATT | 5′FAM |
2-D2S1333* | 12 | CTTTGTCTCCCCAGTTGCTA | TCTGTCATAAACCGTCTGCA | 5′FAM |
3-D2S149* | 13 | GCTTAGTGGGAAATTGTAACTT | GCCTGGCTCCTCTAATACCT | 5′FAM |
4-D3S1768† | 2 | GGTTGCTGCCAAAGATTAGA | CACTGTGATTTGCTGTTGGA | 5′FAM |
5-D4S2365* | 5 | AGTAATTCTTCAACTGCATCACC | ATGCCAAGGATGGTGAGTTA | 5′FAM |
6-D5S1457* | 6 | TAGGTTCTGGGCATGTCTGT | TGCTTGGCACACTTCAGG | 5′FAM |
7-D6S1691† | 4 | AGGACAGAATTTTGCCTC | GCTGCTCCTGTATAAGTAATAAAC | 5′FAM |
8-D6S276† | 4 | TTCCAGTGTATACATCAATCAAATCA | GGGTGCAACTTGTTCCTCCT | 5′FAM |
9-D6S501* | 4 | GCTGGAAACTGATAAGGGCT | GCCACCCTGGCTAAGTTACT | 5′FAM |
10-D7S513† | 3 | AGTGTTTTGAAGGTTGTAGGTTAAT | ATATCTTTCAGGGGAGCAGG | 5′FAM |
11-D7S794† | 3 | GCCAATTCTCCTAACAAATCC | TATGCCCATGTGTTAGGGTT | 5′HEX |
12-D8S1106† | 8 | GCGGCATGTTTTCCTACTTT | TTCTCAGAATTGCTCATAGTGC | 5′HEX |
13-D10S1412† | 9 | GCTCCCTGCGTACTGAGATA | GCACAGTACTTCTCCCTCCA | 5′HEX |
14-D11S925† | 14 | AGAACCAAGGTCGTAAGTCCTG | TTAGACCATTATGGGGGCAA | 5′HEX |
15-D12S364* | 11 | CCAGTCCTGAACAGGC | CCCTGGAAGTCCCATC | 5′HEX |
16-D16S409* | 20 | TGAATCTTACATCCCATCCC | AGTCAGTCTGTCCAGAGGTG | 5′HEX |
17-D17S1300* | 16 | TAGTGTGTATATATGTATGCATGCA | TGCAGATATCTGTCTTTTGGC | 5′HEX |
18-D18S72† | 2 | GCTAGATGACCCAGTTCCC | CAAGAGAGCCCTTTGGTTT | 5′HEX |
19-D19S228* | 19 | AGCTAATATGGCAGAATCTTGAC | GCAGGATCGCAGTGCTT | 5′HEX |
20-MFGT21† | 8 | AACTTCAGTAAGATAAGGACC | CCTGAGGTCTGGACTTTAT | 5′HEX |