Table 1.
CK1δ suppressors are in transcription termination components and unc-44 / Ankyrin.
| gene | # of alleles | cellular process | mutation |
|---|---|---|---|
| kin-20 | 4 | Casein kinase 1 delta | Missense, revertants |
| kin-19 | 1 | Casein kinase 1 alpha | Missense |
| ssup-72 | 5 | Phosphatase for RNAP II CTD | Missense |
| pinn-1 | 3 | Prolyl isomerase for RNAP II CTD | Missense and null |
| pcf-11 | 1 | 3’ pre-mRNA processing | Missense |
| cpsf-4 | 3 | Cleavage polyadenylation factor complex | Missense |
| pfs-2 | 1 | Cleavage polyadenylation factor complex | Missense |
| ctr-9 | 3 | Elongation/termination, Paf1 complex | Missense |
| cdc-73 | 1 | Elongation/termination, Paf1 complex | Splice acceptor, null |
| zfp-3 | 7 | Likely RNA pol II complex | Missense and nulls |
| rpb-2 | 5 | RNAP II subunit B | Missense |
| cdk-8 | 1 | Mediator and RNA pol II complex | Missense |
| unc-44 | 4 | Ankyrin, cytoskeletal | Missense |
| Unconfirmed hits | |||
| zfp-3 | 1 | Likely RNA pol II complex | Intronic mutation |
| swd-2.2 | 1 | Set1/COMPASS complex | Missense |
| pinn-1 | 1 | Prolyl isomerase for RNAP II CTD | Intronic mutation |
The genes identified in the kin-20 suppressor screen. Four kin-20(ox423) revertants and pseudo-revertants were identified, indicating that the screen was approaching saturation. kin-19(ox689) is a predicted hypermorphic mutation of casein kinase 1α, a CK1 isoform closely related to CK1δ. unc-44 alleles are found in the polyadenylation site (ox685, ox832, ox833) and within a conserved intron upstream of the polyadenylation site (ox686). All other mutations are in components of the RNA polymerase II complex, most with functions in transcription termination. The same mutation, pfs-2(R158Q), was identified previously in a neuronal branching suppressor screen (Van Epps et al., 2010).