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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: J Cell Biochem. 2020 Jan 7;121(7):3465–3478. doi: 10.1002/jcb.29621

Figure 5. BHLHE40-AS1 expression induces cytokine signature.

Figure 5.

Panel A: 2.0x105 MCF10A-pBABE and MCF10A-BHLHE40-AS1 cells were plated in 6 well dishes. Total RNA was collected 48h after plating. 100ng RNA from three biological replicates were assessed via Clariom™ D Array and analyzed by the Transcriptome Analysis Console (TAC) software. Panel B: Ingenuity Pathway Analysis identified upstream signaling regulators in the MCF10A overexpression cell line. Upstream regulators were filtered for expression fold change of ±2, ordered by p-value of overlap. Panel C: Expression of indicated cytokines was determined by qRT-PCR from 48h total RNA cell extracts. Bars represent mean ± SD from 3 biological replicates. * p<0.05, two-tailed Student’s t test. Panel D: MCF10A-pBABE and MCF10A-BHLHE40-AS1 cells were immunoblotted with indicated antibodies. Densitometry was performed in ImageJ.