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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: J Cell Biochem. 2020 Jan 7;121(7):3465–3478. doi: 10.1002/jcb.29621

Figure 7. BHLHE40-AS1 interacts with ILF3 to mediate IL-6 production.

Figure 7.

Panel A: 4.5 x107 MCF10A-BHLHE40-AS1 cells were used to pull-down BHLHE40-AS1 RNA with associated proteins with biotinylated DNA oligos as described in methods. qRT-PCR validates successful lncRNA pulldown (left). Associated proteins were separated by SDS-PAGE and immunoblotted for ILF3 (right). Panel B: RNA immunoprecipitation of ILF3 from MCF10A-DCIS cells was performed as described in methods. Associated RNAs were evaluated by qRT-PCR for indicated RNAs. Panel C: 50nM of siRNA targeting ILF3 were transfected into 2.0 x105 MCF10A-BHLHE40-AS1 cells. Total RNA was collected 48 hr later and assessed for indicated gene expression. Bars indicate mean ± SD of 3 biological replicates. * p<0.05, two-tailed Student’s t test.