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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Biochem Pharmacol. 2020 Feb 14;176:113857. doi: 10.1016/j.bcp.2020.113857

Figure 2.

Figure 2.

Effect of NOSH-aspirin on MIA PaCa-2 and BxPC-3 pancreatic cancer cell kinetics. NOSH-aspirin inhibits proliferation by altering cell cycle progression and inducing apoptosis. Cells were treated with vehicle or 50 nM NOSH-aspirin (close to IC50 for cell growth inhibition in both cell lines) and analyzed at the indicated times for proliferation by PCNA antigen expression (Panels A and D, MIA PaCa-2 and BxPC-3 cells respectively); cell cycle phases by PI staining and flow cytometry (Panels B and E, MIA PaCa-2 and BxPC-3 cells respectively); and apoptosis by Annexin V staining and flow cytometry (Panels C and F, MIA PaCa-2 and BxPC-3 cells respectively). In (A), (C), (D), and (F), results are mean ± SEM for 3 different experiments performed in duplicate, *P < 0.05 at the indicated time points. In (B) and (E), results are representative of two different experiments. This study was repeated twice generating results within 10% of those presented here.