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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Biochem Pharmacol. 2020 Feb 14;176:113857. doi: 10.1016/j.bcp.2020.113857

Figure 4.

Figure 4.

NOSH-aspirin induces ROS levels. MIA PaCA-2 cells were treated with 50 nM NOSH-aspirin for 6 h followed by staining with a general ROS probe DCFDA (A) or DHE which detects superoxide anions in cells (B). These experiments were repeated twice and representative histograms are shown. ROS in MIA PaCA-2 cells was also probed with DCFDA following treatment with z-VAD and NOSH-aspirin (C) and similarly examined in BxPC-3 cells (D). The coloring of the backgrounds in the histograms and cleaning of the axis was done by using Adobe Photoshop CS5.