Figure 5.

Adipose-specific Wntless deletion does not influence whole-body metabolism on a normal chow diet. (A) Genetic recombination of Wntless in tissues isolated from Wls^-/- mice. (B–C) Wntless mRNA and protein expression in iWAT and eWAT of Wls^fl/fl and Wls^-/- mice. (D) Wntless mRNA expression in SVF and adipocytes isolated from iWAT and eWAT of Wls^fl/fl and Wls^-/- mice (n = 3). (E) Body composition of 16-week-old Wls^fl/fl and Wls^-/- mice on NCD. (F) Growth curve of 20-week-old Wls^fl/fl and Wls^-/- mice. (G) Glucose tolerance test in 16-week-old Wls^fl/fl and Wls^-/- mice. (H) Insulin tolerance test in 18-week-old mice. (I) Blood glucose concentrations in random-fed and 16 h fasted mice. Serum concentrations of (J) insulin, (K) adiponectin, and (L) triacylglycerols in 20-week-old mice. (M) Tissue weights at time of sacrifice. RNA expression normalized to PPIA. Data shown in E-M are from male mice, n = 5 per group. Data are presented as mean ± S.D. ∗ indicates significance at p < 0.05.