Figure 8.

Wls^-/-mice on HFD have reduced epididymal adipocyte size and decreased DNL gene expression. (A) Representative histological images of H&E-stained tissues from Wls^fl/fl and Wls^-/- mice f 60% HFD for 24 weeks; 200× magnification; scale bar, 100 μm. (B) Adipocyte size quantification of eWAT (400–500 adipocytes/mouse, n = 8 and 7). (C) Wnt target gene expression in adipocytes and SVF isolated from eWAT. (D–E) Lipogenic mRNA and protein expression in isolated eWAT adipocytes. (F) Lipogenic gene expression in eWAT and iWAT from Wls^fl/fl and Wls^-/- mice. Data shown are from male mice. RNA expression normalized to PPIA. Data are presented as mean ± S.D. ∗ indicates significance at p < 0.05.