Fig. 5.

Time courses of MSOT spine signal for representative channels in RENCA-luc mouse. A) Sum image of all channels at final time point, with ROIs used for analysis indicated. T: tumor, S: spine. B, C, D) Time courses of signal in selected channels based on respective filters. B) 700 nm, C) 800 nm, D) 850 nm. Red: sliding window filter, green: $\text{α}$, blue: $\text{α}\text{β}$. The $\text{α}\text{β}$ filter removed a substantial proportion of the noise from the dynamic signal while still accurately following the gas challenge. All channels are shown in Supp. Figures S1 (image comparisons) and S2 (time courses).