FIGURE 3.

Comparison of the effect of halloysite nanotubes loaded with glycerol (HNTs) and prodigiosin-HNTs on malignant (Caco-2, HCT116) and non-malignant cells (MSC, HSF). All cultures were grown in Minimum Essential Medium Eagle – Alpha Modification (α-MEM) with 5% CO₂ in air and humidity at 37°C. The addition of prodigiosin-HNTs during the cultivation of cancer cells (Caco-2, HCT116) led to aberration of their morphology and subsequent detachment from the surface of the dish (Caco-2/prodigiosin-HNTs, HCT116/prodigiosin-HNTs). Glycerol-loaded HNTs which were used as a control did not affect the morphology and viability of all cell types. The presence of prodigiosin-HNTs in the culture medium of non-malignant cells increased the cytoplasm vacuolization of some cells (MSC/prodigiosin-HNTs and HSF/prodigiosin-HNTs inserts).