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. 2020 May 26;11:767. doi: 10.3389/fphar.2020.00767

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Copyright © 2020 Jiang, Wang, Luo, Lin, Feng, Yan, Shi, Zhang, Gu, Cui, Wang, Wang, Zhang and Tan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Polyporus polysaccharide (PPS) reduces alpha-smooth muscle actin (α-SMA) expression in transforming growth factor β1 (TGF-β1) treated human lung fibroblasts (HLFs). (A) Microscopy images are representative morphology analysis in TGF-β1 treated HLFs from four independent experiments (Scale bars, 50 μm). (B) Representative results for detection of alpha-smooth muscle actin (α-SMA) fluorescence in TGF-β1 treated HLFs (Scale bars, 50 μm). DAPI was used as a nuclear counterstain. Quantification of fluorescence intensity normalized to DAPI (right). (C) α-SMA mRNA expression in TGF-β1 treated HLFs (n = 4, per group). (D) Representative Western blot results for α-SMA expression TGF-β1 treated HLFs, normalized against GAPDH. The graph shows the results of the densitometric quantification of the Western blot analysis, which are presented as fold changes as compared with control group. Scatter plots show the mean ± SD quantitative results obtained from three independent studies on 3 different days. ^## = p < 0.01, ^### = p < 0.001 versus Con; * = p < 0.05, ** = p < .001, *** = p < 0.001 versus TGF-β1 treated alone.