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. 2020 May 26;11:767. doi: 10.3389/fphar.2020.00767

Figure 5.

Figure 5

Polyporus polysaccharide (PPS) inhibits extracellular matrix (ECM) proteins expression in transforming growth factor β1 (TGF-β1) treated human lung fibroblasts (HLFs). (A) Levels of Fibronectin, Collagen 1, and Collagen 3 mRNA expression in TGF-β1 treated HLFs (n= 4, per group). Relative mRNA expression was calculated using 2ΔΔ Ct method. The graphs are presented as fold changes as compared with control group. (B) Representative Western blot results for Fibronectin, Collagen 1, and Collagen 3 expression in TGF-β1 treated HLFs (n= 3, per group), normalized against GAPDH. The graph shows the results of the densitometric quantification of the Western blot analysis, which are presented as fold changes as compared with control group. Scatter plots show the mean ± SD quantitative results obtained from three independent studies on 3 different days. ## = p < 0.01, ### = p < 0.001 versus Con; * = p < 0.05, ** = p < 0.01, *** = p < 0.001 versus TGF-β1 treated alone.