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. 2020 May 26;11:767. doi: 10.3389/fphar.2020.00767

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Copyright © 2020 Jiang, Wang, Luo, Lin, Feng, Yan, Shi, Zhang, Gu, Cui, Wang, Wang, Zhang and Tan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Polyporus polysaccharide (PPS) attenuates myofibroblast differentiation via Smad2/3 signaling. (A, B) Representative Western blot results for Smad2 (A) and Smad3 (B) phosphorylation in transforming growth factor β1 (TGF-β1)–treated human lung fibroblasts (HLFs). Scatter plots show the mean ± SD quantitative results obtained from three independent studies. (C) Representative Western blots (left) and semiquantification (n=3, right) for the blots showing levels of TGF-βRI and TGF-βRII expression in TGF‐β1-treated HLFs. ^## = p < 0.01, ^### = p < 0.001 versus Con; * = p < 0.05, ** = p < 0.01 versus TGF-β1 treated alone. Western blots were normalized against GAPDH. The graph shows the results of the densitometric quantification of the Western blot analysis, which are presented as fold changes as compared with control group.