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. 2020 May 26;13:88. doi: 10.3389/fnmol.2020.00088

Figure 1.

Figure 1

Protein expression profiles of GABA components in Fragile X Mental Retardation 1 (FMR1)-knockout (KO) medial prefrontal cortex (mPFC). Western blot analysis of selected GABAA subunits (Ai,Bi), the two GABAB subtypes (Aii,Bii), gephyrin (Aiii,Biii), and GABA transporter 1 (Aiv,Biv), in mPFC, enriched synaptosomal fractions from Fmr1-KO and wildtype (WT) age-matched littermates. (A) No difference in protein expression in any of the GABA machinery components tested was found in prepubescent Fmr1-KO mPFC. (B) GABAA receptor subunit α2 expression was enhanced during adolescence (Bi, WT(n = 7) = 0.93 ± 0.16, KO(n = 8) = 1.18 ± 0.25, p = 0.04), while expression of GABAB receptor subtype B1 was found reduced during the same postnatal developmental period (Bii, WT(n = 8) = 0.99 ± 0.31, KO(n = 8) = 0.77 ± 0.27, p = 0.03). Depending on the sample distributions, differences between genotypes were assessed with either the Student’s t-test or the Mann–Whitney U test. For all panels, asterisks denote statistical significance of *p < 0.05. Error bars represent SD.