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. 2020 Jun 1;11(6):408. doi: 10.1038/s41419-020-2621-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Venn diagrams showing the number of potential miR-130b-3p targets. b RT-qPCR analysis of six potential miR-130b-3p target genes in MB cells with miR-130b-3p overexpressed. c The potential binding sites of miR-130b-3p within the SIK1 mRNA 3′-UTR and 3′-mUTR. Luciferase reporter gene assays were used to analyze the effect of miR-130b-3p on luciferase activity. d SIK1 protein levels were detected through western blotting in MB cells transfected with miR-130b-3p mimic. NC negative control. Data represent means ± SEMs from three independent experiments. *p < 0.05, **p < 0.01.