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. 2020 Jun 1;11(6):408. doi: 10.1038/s41419-020-2621-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a CCK-8 assay in MB cells treated with SIK1 inhibitor. b The efficiency of si-SIK1 knockdown was detected by RT-qPCR. c The proliferative ability of MB cells transfected with si-SIK1 or NC was tested through CCK-8. d Cell apoptosis was detected through flow cytometry in MB cells treated with si-SIK1 or negative control. e Cell migration was detected through wound-healing assay in MB cells transfected with si-SIK1 (original magnifications ×200). f Cell invasion analysis was performed through transwell invasion assay in MB cells transfected with si-SIK1 (original magnifications ×200). Data represent means ± SEMs from three independent experiments. *p < 0.05, **p < 0.01.