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. 2020 Jun 1;10:8858. doi: 10.1038/s41598-020-65649-y

Figure 3.

Figure 3

Propofol affects miR-149-5p, miR-760, mammospheres and EMT. Parental or Trastuzumab-resistant (Tr-R) SKBR3 or HTB-20 cells were treated with either DMSO vehicle or 5 μg/ml propofol for 96 h. Total mRNA was then collected from the cells and miR-149-5p/miR-760 quantitated by quantitative RT-PCR. U6 was used as an internal control for miRNA expression. Mammospheres generated, as described in Methods, were counted under different experimental conditions and levels of EMT marker, snail, quantitated by quantitative RT-PCR. For mRNAs’ internal control and normalization, we used gene expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). *p < 0.05 and #p < 0.01, compared to vehicle.