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. 2020 Jun 1;10:8858. doi: 10.1038/s41598-020-65649-y

Figure 5.

Figure 5

In vivo study. (A) Parental or trastuzumab-resistant (Tr-R) SKBR3 cells were injected intra-venously in athymic mice, as described in Methods. The propofol treated mice were in two different groups – either injected with cells that were transfected with non-scrambled control (propofol) or injected with cells transfected with anti-miR-149-5p oligos (propofol + Anti-miR). Lung metastatic nodules (Lung Mets) were counted in all animals (n = 8 each in each group). (B) The extracted modules were weighed and exactly equal amounts (50 mg) were subjected to RNA isolation and subsequent culture in culture dishes. Cell culture supernatant was collected and ELISA was performed to quantitate levels of IL-6. #p < 0.01, compared to vehicle and $p < 0.01, compared to propofol alone.