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. 2020 Jun 1;11:2699. doi: 10.1038/s41467-020-16526-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a MHC expression in Compound-Het animals display significant changes in MHC distribution. A shift away from type IIB and toward slower myosin isoforms (either type IIA/X or type I) is observed in most muscle types (an exception is the tibialis m.). Top representative MHC gel images; bottom analyzed results. The control (CTRL) is a pooled homogenate of TC and Soleus muscle, revealing the 3 isoforms that can be discerned: type I, IIA/X and IIB (from bottom to top). b Example cross-sections stained with antibodies that are MHC-type specific (left), minFeret diameter (right, top) and number of fibers (right, bottom) in EDL muscle. MinFeret is increased in type I fibers and reduced in IIX. The number of type I fibers number is increased (only 3 type I fibers were found in WT mice) and the number of type IIB fibers is reduced. Scale bar: 100 μm. c Example cross-sections stained with antibodies that are MHC-type specific (left), minFeret diameter (right, top) and number of fibers (right, bottom) in soleus muscle. There is a reduction in type IIX and IIB minFeret and an increase in the number of type I and a reduced number of type IIA fibers. Scale bar: 100 μm. a–c: Two-way ANOVA (no matching) with a posthoc multiple testing comparison with multiple testing correction (Sidak). *p < 0.05, **p < 0.01; ***p < 0.001; ****p < 0.0001. Number of mice in a: WT: 9, Compound-Het: 7. Number of mice in b and c: WT: 6, Compound-Het: 7. Bar values are mean ± SEM. Source data are provided as a Source Data file.