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. 2020 Jun 1;10:8879. doi: 10.1038/s41598-020-65817-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of proximal tubule barriers. (A) The formation of a proximal tubule monolayer on a permeable support insert. (B) CK8/18 (green) (C) CK8/18 (green), cross section (D) ace-tubulin (green) (E) MRP-2 (red) (F) Na + -K + -ATPase (red) (G) ZO-1 (red) (H) Claudin-10 (red) (I) Ki67 (red). Nuclei were stained with DAPI (blue). Scale bars B, D, and E: 100 μm; C, F, G, H and I: 50 μm. qRT-PCR analysis of (J) MRP-2 (K) P-gp (L) Ki67 gene expression. The fold changes in mRNA expression of the barriers at the endpoint are represented relative to barriers newly formed; data are the mean ± SEM, experiments were performed in triplicates.