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. 2020 Jun 1;3:276. doi: 10.1038/s42003-020-0999-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Lem2 fragments indicated on the left were expressed in lem2Δlnp1Δ cells. Diagram of these fragments is shown on the right. Fivefold serially diluted cells were spotted on EMMG plates, and growth of these cells was observed after 3 days. b Lnp1 fragments indicated on the left were expressed in lem2Δlnp1Δ cells. Diagram of these fragments is shown on the right. The asterisk indicates the mutation site of G2A. Fivefold serially diluted cells were spotted on EMMG plates, and growth of these cells was observed after 3 days. c Localization of Lnp1 fragments GFP-tagged Lnp1 fragments (green) and cortical ER membrane marker Rtn1-mRFP (magenta) were expressed in wild-type cells. Scale bar represents 5 μm.