TABLE 5.

Significant differences in community structure in the fecal microbiota of Holstein heifers among the three groups.

Group	Anosim		MRPP
	R-value	P-value	A-value	Observed delta	Expected delta	P-value
CN-LC	0.4905	0.001	0.09894	0.2821	0.3131	0.001
CN-HC	0.3245	0.001	0.0739	0.306	0.3304	0.003
LC-HC	0.2366	0.008	0.0746	0.3252	0.3515	0.003

Anosim, analysis of similarity; MRPP, multiresponse permutation procedure. The R-value ranged from −1 to 1, and an R-value > 0 showed a significant difference in the between-group population compared with those in the within-group population, as well as the A-value (analyzed by MRPP, multiresponse permutation procedure). The P-values indicated the significant differences at the levels of P < 0.05 or P < 0.01. CN, control no HTR; LC, 5% fermented HTRs replaced corn silage; HC, 10% fermented HTRs replaced corn silage.