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. 2020 Jun 1;3:277. doi: 10.1038/s42003-020-1000-7

Fig. 4. TA and DOP did not inhibit the HaCaT cell proliferation and the increase of intracellular Ca++.

Fig. 4

a HaCaT cells proliferations were measured using MTT assay. The presence of EPI (25 µg/ml) increased the cell proliferations compared to control. The addition of TAs and DOP (25 µg/ml) showed no significant effect. b The intracellular Ca++ was measured using fluorescence-based assay. HaCaT cells was incubated in the presence of EPI (25 µg/ml) alone, and EPI with TA, DOP, and ICI. EPI increased the intracellular Ca++ level with and without TA and DOP at 3 min ICI nullified the effect of EPI both in terms of cells proliferation and increase in intracellular Ca++. For all graphs, each data point is the mean value ± SEM from three independent replicates, *p < 0.05; **p < 0.01; and ***p < 0.001, data were analyzed using Student's t test. Source data are provided as a Supplementary Data 1 file.