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. 2020 Jun 1;10:8875. doi: 10.1038/s41598-020-64947-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OSCC cell features. (A) OSCC cells (black arrow) and fibroblasts (white arrow) direct outgrowth from the OSCC specimens. (B) OSCCs after a 15-day culture. (C) Brown staining was positive in the cytoplasm of OSCC cells detected by immunohistochemical staining for keratin. (D) Minimal brown staining was observed in the cytoplasm of OSCC cells in the blank control group. (E,F) CD133⁺ OSCC cells formed in adipose tissue observed by microscopy following Oil Red O staining. (G,H) Calcified nodules formed by CD133⁺ OSCC cells stained with Alizarin Red.