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. 2020 Jun 1;10:8875. doi: 10.1038/s41598-020-64947-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of CD133⁺ OSCC cells. (A) CD133⁺ cells purified up to 94.33% using MACS. Expression of CD133 in 0.41%±0.06 of primary OSCC cells and detected by flow cytometry. (B) Cell cycle analysis of CD133⁺ cells revealing that 93.39% of CD133⁺ OSCCs cells were in the G0/G1 phase. (C) Immunofluorescence detection of CD133 expression on OSCC cells and primary OSCC cells. All images were obtained at 400× magnification. (D) The normalized mean IOD of CD133⁺ OSCC cells was higher than that of primary OSCC cells. Data were analysed by 2-tailed t test. **P < 0.01.