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. 2020 Jun 1;10:8875. doi: 10.1038/s41598-020-64947-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo tumourigenic assay. (A,B) CD133⁺ OSCC cells showed more aggressive tumour stem cell behaviour. Data analysed by 2-tailed t test. *P < 0.01. (C) The transplanted tumour was identified as well-differentiated SCC according to HE staining. Cancer pearls observed in the specimen (C, black arrow). (D) Cytokeratin detected in the CD133⁺-specific transplanted tumour according to immunohistochemical staining (black arrow).