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. 2020 Jun 1;9(5):58. doi: 10.1038/s41389-020-0241-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a IHC analysis of PNO1 expression in tissue microarrays of LUAD specimens (scale bar, 200 μm or 100 μm). b The proportions of LUAD patients with different PNO1 expression levels. c Kaplan–Meier survival curve showing the correlation between the PNO1 IHC score and OS of LUAD patients. d IHC analysis of PNO1 expression in tissue microarrays of SCLC specimens (scale bar, 200 μm or 100 μm). e The proportions of SCLC patients with different PNO1 expression levels. f Kaplan–Meier survival curve analysis showing the correlation between the PNO1 IHC score and OS in SCLC patients. g, h Kaplan–Meier survival curve analysis showing the correlations between LN metastasis or TNM stage and OS in LUAD patients. i Forest plots showing the multivariate risk factors of LUAD patients. j Correlation analysis between the PNO1 IHC score and tumor size. k Kaplan-Meier survival curve showing the correlation between the PNO1 IHC score and OS in a subgroup of LUAD patients (tumor size >3 cm). l Correlation analysis between the PNO1 IHC score and TNM stage. m Kaplan–Meier survival curve showing the correlation between the PNO1 IHC score and OS in a subgroup of LUAD patients (TNM stage III). n, o Western Blotting and RT-PCR showing the differences in the PNO1 protein and mRNA levels between eight pairs of LUAD tissue and adjacent nonmalignant tissue. nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.