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. 2020 Jun 1;9(5):58. doi: 10.1038/s41389-020-0241-0

Fig. 2. Knocking down PNO1 expression suppresses LUAD cell metastasis and proliferation in vitro.

Fig. 2

a, b The construction of PNO1-control (sh-Ctrl) and PNO1-knockdown (sh-PNO1) A549 and NCI-H1299 LUAD cell lines. c, d The chemotaxis potential and invasive ability of the A549 sh-Ctrl and sh-PNO1 groups (scale bar, 1.0 mm). e, f The chemotaxis potential and invasive ability of the NCI-H1299 sh-Ctrl and sh-PNO1 groups (scale bar, 1.0 mm). g, h Wound-healing assay comparing the migration distance between the sh-Ctrl and sh-PNO1 groups of A549 or NCI-H1299 cells. i, j MTT assay showing the proliferative abilities in the sh-Ctrl and sh-PNO1 groups of A549 or NCI-H299 cells. k, l Comparison of the proliferative potential between the sh-Ctrl and sh-PNO1 groups of A549 or NCI-H299 cells by a colony formation assay. m, n Cell apoptosis assay revealing the proportions of apoptotic A549 and NCI-H1299 cells in the sh-PNO1 and sh-Ctrl groups. *P < 0.05, **P < 0.01, ***P < 0.001.